Composite

Part:BBa_K208040:Design

Designed by: USU iGEM 2009   Group: iGEM09_Utah_State   (2009-10-20)

Lambda cL Reg. Promoter/RBS/PelB/PhaP/Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 129
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silver Fusion-friendly. Lambda cL Reg. promoter, RBS, PelB secretion tag, PhaP coding, and two terminators in pSB1AK3 vector. Meant for PhaP secretion.

Source

Composite

References